质粒名称: | pDD04neo pmyo2::gfp |
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出品公司: | 爱迪基因 |
目录编号: | 26393 |
存储实验室: | Denis Dupuy |
载体骨架基本信息 |
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载体名称: | pBS |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 细菌表达,蠕虫表达 |
筛选标记: | 新霉素(G418) |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | -- |
物种来源: | C. elegans (nematode) |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pDD04neo pmyo2::gfp was a gift from Denis Dupuy (Addgene plasmid # 26393 ; http://n2t.net/addgene:26393 ; RRID:Addgene_26393) |
参考文献部分: | An antibiotic selection marker for nematode transgenesis. Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, Baillie D, Vidal M, Dupuy D. Nat Methods. 2010 Aug 22. ():. 10.1038/nmeth.1494 PubMed 20729841 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDD04neo pmyo2::gfp 9672 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDD04neo pmyo2::gfp SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9672) AUTHORS Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, Baillie D, Vidal M, Dupuy D TITLE An antibiotic selection marker for nematode transgenesis. JOURNAL Nat Methods. 2010 Aug 22. ():. PUBMED 20729841 REFERENCE 2 (bases 1 to 9672) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9672 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 24..128 /gene="bla" /label=AmpR promoter CDS 129..989 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(347..366) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1160..1748 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1649..1668 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1902..1919 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2036..2057 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2072..2102 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2110..2126 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2115..2137 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2134..2150 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2134..2150 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2169..2189 /label=T3 /note="T3 promoter, forward primer" promoter 2171..2189 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind 2226..2242 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 2226..2242 /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(2276..2292) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2277..2293) /label=pBluescriptKS /note="For pBluescript vector" CDS complement(3001..3795) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3112..3131) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 3722..3741 /label=Neo-R /note="Neomycin resistance gene, reverse primer" protein_bind 4632..4652 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" protein_bind 7165..7189 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(7214..7242) /label=GFP-R /note="GFP, reverse primer. Does NOT anneal to EGFP" primer_bind 7990..8010 /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" protein_bind complement(8942..8966) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(9028..9047) /label=T7 /note="T7 promoter, forward primer" promoter complement(9029..9047) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" rep_origin 9215..9670 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9302..9321) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9512..9533 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg 2221 gccgctctag aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata 2281 ccgtcgacct cgagaaacag ttatgtttgg tatattggga atgtattctg tcatttaagg 2341 caaaaacccc atagacacta ctccactttc aaattatcaa cttctactca ccttccactg 2401 agcctcaaac ccaaaccttc ttccgatctt tcttgcatcg tgctcatcaa tacttgtgat 2461 tttagcaaga aaaacgccgt cctcgaaact cttgacaaac aataggggaa acccccaaaa 2521 agcaaaagca ggaaaattaa tttcataaac tttcacttga ttgaaaaatt ccaaaaatac 2581 tccaaaactt tttcacgatg agcatgattt gacgtcatga gaggcccaga cgtgcgaaga 2641 aataaaaatt gcggtcataa actgaaacgt aacatatgat aaggtatttt gtgtgcggtt 2701 ttttctatga tgtttcaaaa aaaatttatc agaagtaaaa aaaacataag aagtgtacac 2761 agaacattgt gtagattttg ggatagaatt attattaatg gggggaggga gcacaatttt 2821 tttcgacttt ttattacgaa ttaattctat ttttgaatct acacaatttc attgttagag 2881 gtgacttaaa agaagctaaa aaacaaagaa attaagaaga agtttttttg ataataacaa 2941 aaataggggg tgggagcaca gggagaaaga gcatgtaggg atgttgaaga gtaattggac 3001 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat 3061 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg 3121 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa 3181 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac 3241 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc 3301 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt 3361 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag 3421 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg 3481 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc 3541 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg 3601 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac 3661 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg 3721 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc 3781 atcttgttca atcattttta ttccacttgt tgagcggggc tggctgaaaa tatgtgaatt 3841 gagaaaataa atagtatatt tgaaagaagg gaagacgaga taaacggaaa aacacgattg 3901 cggaacatta cgaaaaattt cgaaatttat tattttttta ataacatttg tttaccggcc 3961 ggacaagaca aaccaacttc gaaacgagga ataaatcaaa tttcatattt aaaatgaatc 4021 aaaacccatg attaaacgaa atgacaaaaa tcgataacgt gtatagaacg aactcaagct 4081 gaaccgagaa ccgagaaatt cagggttttt agtattttat tatttatttt agttttattt 4141 aagctgataa tttcgaaacg ttatgatttg aataaaaatc gtggaatatt gatattttcc 4201 caaaaattcc acttggtaga atcacaaatc taggcaaatt ggccgttttc tatcacaaat 4261 tacgagcgaa aactacaaat aatgcaattt ttatatactt ttctaactaa aaacctgaaa 4321 aaaatatcga aaaaatcggg aaaacagttg aaaaacggga aaaaaagggt taaaatttta 4381 ggcccagcaa tttcatcgcc ttccgcaaac cgtgcggtgg ggcggagaat gtaatggtgc 4441 ggcgcggttt tcggagtgtc gtagcgcgct gtgtttatcg taaatcggcg cgaactcttt 4501 tttgaagcaa ttttttttgg tttttattga aagttagtct caaaactcgt attattttaa 4561 aattttttta ttaaactaaa atttagaaat gggcccttgg atcctctaga gcggccgccg 4621 actagtgata tcaactttgt atagaaaagt tgaaggtctc ccagaacgtg aataaaagct 4681 gctcggacgt tgatgttatc gccgttgcca ccaccatgag agtgaccatg agagcttccg 4741 cctccgtgag aatgtgaatg accaccaaag tacaacaatg caagcatgct taaattttga 4801 ttttgaatat caattaagat acatatgtca cattagattt gtttcgaact caaaaaaatt 4861 tctttgaaaa ccgtaagtcc gtgacagcct aatttttagt taaaagaaaa taacatggac 4921 agacattttt ctgacatgtc aacattcctg gggctcccac ccggataaaa gttcatgcca 4981 aatatcgatt tcaaatcgaa gtaaaaaagt gtataccagt taataaagct atcagactcg 5041 tttcataaaa acacttacac caagtttaca accactccaa gagcggctgt caaagccata 5101 attcctcctt ctacttcata atctccgctg acaattctca taattgcgag aacaactaat 5161 actccagtga ctatccaaat aaggaaaact gagaaaaatg ctcccaaaac ttctgaaatt 5221 catttcaaac tatgtcgctc aattcttcca actacccacc ggctctatga aacccaaagc 5281 tcattttttg cgatggtggc cgtcttgcaa tgtacagaga aaacagcgaa atgagcactg 5341 acgcaaaatc agtgagcaag tgtgcggcat ctgtgacaat tgcgagtgag cccgcgagaa 5401 caccaccaat aacttcgcaa accataaaaa ataggcagag cacgacggtg agccataaaa 5461 ttcgcgttgc tgaaacaaat agtttgaaga aggtttcatt aggactcgag tttggcagaa 5521 attttaaaag caatacctct ccgattgcta tcatgtgaat ctgttgagtc agcttcatcg 5581 tggcaatgaa agttatcata ggtgactgtg gtttttctac cttcgtctgt attataaatg 5641 tgaggaactc tgtaatacaa aaagatctca tgttttttct ttggtaatac gtcatagaag 5701 cacaggaaat cacaactctg atataaacga acatcaacta catcacaaaa ctaacccatt 5761 ttcaagtagt ggtgctcgtt cagaaatcat tatggattat ggagaaaagg aaaccgatta 5821 ggccaatcga ttttgttttg atagaattga agacgcagaa taattgtgtt gtgtatagtg 5881 tacgagaaaa tggagcaact tgtatatggg cactgttatc attattgaat agcgtgcgga 5941 ggtttagaga aggaagttgt gtgttatcaa tcataaacta gaacatgtct cgaggcattt 6001 gaattggggg tggtggacag taactgtctg taataataat tactcctgac caggttgcaa 6061 ttcgagtttt gataagcata attatacctt gtacattgtg ggttttgtgc tgtggacgtt 6121 ttattgtgac atccccataa gctacaagaa caaaaatgaa atgtaaaagt attgaaaacg 6181 tcgtacattt tatatctgag tagtatcctt tgctttaaat gtccataaaa ctaattttat 6241 aatcaataaa acaacgtttg taaatcaact gagtttacaa gtagagacat tgagggatac 6301 tttcactatg ctaaagtgat aatcgaccaa ataataactt cactttggta tttattcctg 6361 tcttataaat gttatgtatg aattaaattc atatgcatat ggctcactct gacaattttt 6421 aataatcttc cagatcaata ttgactaccg atgcgggtgg tcttttgctt tgaattctgc 6481 tgaactttac accccgaaca gcaatgtgtg cttcagccta aaaaaaagta agtgtgttaa 6541 tcagtgcccc cgattcttca ttttttgccc ctctctcccg tttcgtcggc aaaagaagag 6601 aaaataaaga taagtctcaa gataggttgg taatcgctaa agtggttgtg tggataagag 6661 tagcaaaatg gcaggaagag cactttgcgc gcacacactg tactcattgt tctggataaa 6721 attctctcgt tgtttgccgt cggatgtctg cctctctgca ttgagccggc ttcttcacta 6781 tctttagtta acctaaaatg ccgtttcttt tctcgtatcc ccactatccc gttgaggttc 6841 tctgctctct tcgctcccta ccgccagcga gcaactatcc gtgggggcgc cttgctcgga 6901 agatgggggg gaagaaagaa gatttttgct atttgcactt gagaaagaga cttttcctgc 6961 gtcgatggtt agagaacagt gtgcagacac ttttcagcta cctagaatta caattggata 7021 tccccgcctc ccaatccacc cacccaggga aaaagaaggg ctcgccgaaa atcaaagtta 7081 tctccaggct cgcgcatccc accgagcggt tgacttctct ccaccacttt tcattttaac 7141 cctcgatcgt cagacacaga aatgacaagt ttgtacaaaa aagcagagta aaggagaaga 7201 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa 7261 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt 7321 tatttgcact actggaaaac tacctgttcc atgggtaagt ttaaacatat atatactaac 7381 taaccctgat tatttaaatt ttcagccaac acttgtcact actttctgtt atggtgttca 7441 atgcttctcg agatacccag atcatatgaa acggcatgac tttttcaaga gtgccatgcc 7501 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg 7561 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag gtgctgaagt 7621 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga 7681 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat 7741 catggcagac aaacaaaaga atggaatcaa agttgtaagt ttaaacatga ttttactaac 7801 taactaatct gatttaaatt ttcagaactt caaaattaga cacaacattg aagatggaag 7861 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt 7921 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag 7981 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga 8041 actatacaaa tagcattcgt agaattccaa ctgagcgccg gtcgctacca ttaccaactt 8101 gtctggtgtc aaaaataata ggggccgctg tcatcagagt aagtttaaac tgagttctac 8161 taactaacga gtaatattta aattttcagc atctcgcgcc cgtgcctctg acttctaagt 8221 ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt 8281 gttattatca aaaaaacttc ttcttaattt ctttgttttt tagcttcttt taagtcacct 8341 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa 8401 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt 8461 gtacacttct tatgtttttt ttacttctga taaatttttt ttgaaacatc atagaaaaaa 8521 ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc 8581 ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt 8641 atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aatgtccttt cttttgcttt 8701 ttgggggttt cccctattgt ttgtcaagag tttcgaggac ggcgtttttc ttgctaaaat 8761 cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca 8821 gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc 8881 ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt 8941 aacccagctt tcttgtacaa agttgatatc tcgacccggg aattccggac cggtaccagc 9001 tttgggcccg gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcgctg 9061 caacattttg ctgccggtca tgactgggaa aaccctggcg ttacccaact taatcgcctt 9121 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 9181 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc 9241 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 9301 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 9361 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 9421 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 9481 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 9541 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 9601 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 9661 cttacaattt ag //